abstract：:The prognostic value of DNA ploidy and proliferative index (PI) are well established in many cancers, but their significance in childhood rhabdomyosarcoma (RMS) is unclear. We studied the DNA content and PI of 45 cases of childhood RMS obtained retrospectively. DNA histograms were hyperdiploid in 30 cases (67%), diplo...

journal_title：American journal of clinical pathology

authors： San Miguel-Fraile P,Carrillo-Gijón R,Rodriguez-Peralto JL,Badiola IA

更新日期：2004-03-01 00:00:00

abstract：:We evaluated a comprehensive deidentification engine at the University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, that uses a complex set of rules, dictionaries, pattern-matching algorithms, and the Unified Medical Language System to identify and replace identifying text in clinical reports while preserving ...

journal_title：American journal of clinical pathology

authors： Gupta D,Saul M,Gilbertson J

更新日期：2004-02-01 00:00:00

abstract：:We evaluated the immunohistochemical profile and specificity of CD138 reactivity in 238 specimens from hematopoietic and nonhematopoietic neoplasms. In 91 bone marrow biopsies, CD138 reactivity was observed for nonneoplastic plasma cells, neoplastic plasma cells in multiple myeloma cases (43/43), and the plasmacytic c...

journal_title：American journal of clinical pathology

authors： O'Connell FP,Pinkus JL,Pinkus GS

更新日期：2004-02-01 00:00:00

abstract：:The efficacy of a noninvasive hemoglobin monitoring device (Astrin, Sysmex, Kobe, Japan) was evaluated for healthy volunteers and for patients with hematologic disorders. At the same time, the effects of WBC counts on noninvasive monitoring were studied by clinical evaluation and in ex vivo experiments. The hemoglobin...

journal_title：American journal of clinical pathology

authors： Saigo K,Imoto S,Hashimoto M,Mito H,Moriya J,Chinzei T,Kubota Y,Numada S,Ozawa T,Kumagai S

更新日期：2004-01-01 00:00:00

abstract：:Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. It has become apparent that rFVIIa is useful in controlling bleeding in a variety of clinical situations. This review attempts to collate and summarize the nonhemophilia...

journal_title：American journal of clinical pathology

authors： Midathada MV,Mehta P,Waner M,Fink LM

更新日期：2004-01-01 00:00:00

abstract：:Since the introduction of the triple screen, the emergence of additional analytes found to be associated with trisomy 21 pregnancies has challenged the clinical pathology and obstetric communities to determine which combination provides the best performance characteristics as a screening test. The cost, practicality, ...

journal_title：American journal of clinical pathology

authors： Baliff JP,Mooney RA

更新日期：2003-12-01 00:00:00

abstract：:We undertook a retrospective analysis of colonic spirochetosis in 14 cases: females, 3; males, 11; children, 4; adults, 10. Two men had HIV infections. All children and both HIV-infected men had abdominal complaints, diarrhea, or both. Most other adults underwent colonoscopy for polyp screening (n = 4) or follow-up of...

journal_title：American journal of clinical pathology

authors： Koteish A,Kannangai R,Abraham SC,Torbenson M

更新日期：2003-12-01 00:00:00

abstract：:To evaluate the etiologic role of human papillomavirus (HPV) in oral carcinogenesis, DNA samples were purified from 103 oral squamous cell carcinoma (OSCC) and 30 normal oral mucosal (NOM) specimens. A nested polymerase chain reaction, DNA sequencing, and gene-chip HPV typing were used to identify multiple HPV types i...

journal_title：American journal of clinical pathology

authors： Chang JY,Lin MC,Chiang CP

更新日期：2003-12-01 00:00:00

abstract：:The volume of data contained in a data warehouse represents a potential resource to provide the basis for detailed and specific reference intervals. Routine chemistry panel testing data were derived from an outreach laboratory patient population of 438,180 people and then screened by multiple data filters to identify ...

journal_title：American journal of clinical pathology

authors： Bock BJ,Dolan CT,Miller GC,Fitter WF,Hartsell BD,Crowson AN,Sheehan WW,Williams JD

更新日期：2003-11-01 00:00:00

abstract：:We studied the beta-catenin immunohistochemical profile in tumors expressing shadow cells: pilomatricoma, 10 cases; calcifying odontogenic cyst, 6 cases; and craniopharyngioma, 9 cases. There was strong membranous, cytoplasmic, and nuclear staining of the immature basaloid cells in all of these tumors. Shadow cells we...

journal_title：American journal of clinical pathology

authors： Hassanein AM,Glanz SM,Kessler HP,Eskin TA,Liu C

更新日期：2003-11-01 00:00:00

abstract：:The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of...

journal_title：American journal of clinical pathology

authors： Rarok AA,Huitema MG,van der Leij MJ,van der Geld YM,Berthold H,Schmitt J,Stegeman CA,Limburg PC,Kallenberg CG

更新日期：2003-10-01 00:00:00

abstract：:Reduction mammaplasty (RM) is a common surgical procedure that yields a variable amount of tissue for pathologic examination. Occult breast carcinomas are detected rarely in these specimens. We evaluated the pathologic findings in RM specimens performed in our institution during an 11.5-year period (July 1989 to Decem...

journal_title：American journal of clinical pathology

authors： Ishag MT,Bashinsky DY,Beliaeva IV,Niemann TH,Marsh WL Jr

更新日期：2003-09-01 00:00:00

abstract：:To assess the diagnostic accuracy of margin evaluation of melanocytic lesions using en face frozen sections compared with standard paraffin-embedded sections, we studied 2 sets of lesions in which en face frozen sections were used for analysis of surgical margins (13 from malignant melanomas [MMs] and 10 from nonmelan...

journal_title：American journal of clinical pathology

authors： Prieto VG,Argenyi ZB,Barnhill RL,Duray PH,Elenitsas R,From L,Guitart J,Horenstein MG,Ming ME,Piepkorn MW,Rabkin MS,Reed JA,Selim MA,Trotter MJ,Johnson MM,Shea CR

更新日期：2003-08-01 00:00:00

abstract：:We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage cl...

journal_title：American journal of clinical pathology

authors： Chandler WL,Ferrell C,Lee J,Tun T,Kha H

更新日期：2003-07-01 00:00:00

abstract：:Platelet-derived growth factors (PDGFs) exert their biologic function by binding to 3 different tyrosine kinase receptor isoforms. Especially the PDGF-alpha alpha receptor binds PDGF proteins with high specificity, which results in growth stimulation. The expression of the receptor and its ligand was studied in human ...

journal_title：American journal of clinical pathology

authors： Sulzbacher I,Birner P,Träxler M,Marberger M,Haitel A

更新日期：2003-07-01 00:00:00

abstract：:Although some studies have examined the expression of aberrant markers such as CD2, CD7, CD10, CD13, CD33, and CD34 on B cells in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), a uniform multiparametric analysis of the frequency of expression of these markers using stringent criteria is lacking. By...

journal_title：American journal of clinical pathology

authors： Kampalath B,Barcos MP,Stewart C

更新日期：2003-06-01 00:00:00

abstract：:Acute exacerbations of idiopathic pulmonary fibrosis/cryptogenic fibrosing alveolitis (IPF/CFA) are rare and typically terminal events, but their relationship to underlying patterns of idiopathic interstitial pneumonias is unknown. We reviewed autopsy material from patients who died of diffuse alveolar damage in the c...

journal_title：American journal of clinical pathology

authors： Rice AJ,Wells AU,Bouros D,du Bois RM,Hansell DM,Polychronopoulos V,Vassilakis D,Kerr JR,Evans TW,Nicholson AG

更新日期：2003-05-01 00:00:00

abstract：:The recent clinical and commercial success of anticancer antibodies such as rituximab and trastuzumab has created great interest in antibody-based therapeutics for hematopoietic malignant neoplasms and solid tumors. Given the likelihood of lower toxic effects of antibodies that target tumor cells and have limited impa...

journal_title：American journal of clinical pathology

authors： Ross JS,Gray K,Gray GS,Worland PJ,Rolfe M

更新日期：2003-04-01 00:00:00

abstract：:The aim of this study was to evaluate the histopathologic features and the expression of angiogenesis-related markers in primary tumors and metastatic lymph nodes of oral squamous cell carcinomas (SCCs) with multiple lymph node involvement in comparison with oral SCCs without nodal metastasis. The protein levels of th...

journal_title：American journal of clinical pathology

authors： Nikitakis NG,Rivera H,Lopes MA,Siavash H,Reynolds MA,Ord RA,Sauk JJ

更新日期：2003-04-01 00:00:00

abstract：:Determination of HER-2/neu oncogene amplification has become clinically important in the present management of breast cancer and may have important applications in other areas of clinical oncology and scientific research. In situ hybridization is an extremely accurate and sensitive technique for assessing amplificatio...

journal_title：American journal of clinical pathology

authors： Gupta D,Middleton LP,Whitaker MJ,Abrams J

更新日期：2003-03-01 00:00:00

abstract：:We studied the antigen expression profiles of 19 anaplastic large cell lymphoma (ALCL) cases by multiparameter flow cytometry. The neoplastic cells expressed CD45, HLA-DR, and CD30 in all cases. At least 1 T cell-associated antigen was expressed in each case (CD2, 12/17 [71%]; CD4, 12/19 [63%]; CD3, 6/19 [32%]; CD7, 6...

journal_title：American journal of clinical pathology

authors： Juco J,Holden JT,Mann KP,Kelley LG,Li S

更新日期：2003-02-01 00:00:00

abstract：:Fresh open lung biopsy material from 57 patients was incubated with fluoresceinated complement and immunoglobulin antisera. An indirect immunofluorescent assay using neonatal lung as substrate was conducted as well. Direct immunofluorescent patterns could be categorized into interalveolar septal capillary deposition, ...

journal_title：American journal of clinical pathology

authors： Magro CM,Morrison C,Pope-Harman A,Rothrauff SK,Ross P Jr

更新日期：2003-02-01 00:00:00

abstract：:The results from fetal-maternal hemorrhage (FMH) detection and quantitation external quality assessment surveys conducted in Ontario indicate that the rosette test had a sensitivity and specificity for an FMH of more than 10 mL of 1.0 and 0.75, respectively, compared with 0.96 and 0.92, respectively, for acid elution....

journal_title：American journal of clinical pathology

authors： Lafferty JD,Raby A,Crawford L,Linkins LA,Richardson H,Crowther M

更新日期：2003-01-01 00:00:00

abstract：:The expression of bcl-2, CD10, and CD20 was examined by multicolor flow cytometry in 78 samples including lymph node or other tissue biopsy specimens containing follicular lymphoma (FL; n = 17), reactive hyperplasia (RH; n = 28), or other malignant lymphomas (n = 20), as well as bone marrow aspirates (n = 13). The pre...

journal_title：American journal of clinical pathology

authors： Cook JR,Craig FE,Swerdlow SH

更新日期：2003-01-01 00:00:00

abstract：:Worldwide, infection with hepatitis viruses remains a major clinical and public heath concern. The laboratory is an integral part of the diagnosis of these infections since many of the manifestations are nonspecific, and the various hepatitis viruses show overlapping symptomatology. In this article, we discuss the lab...

journal_title：American journal of clinical pathology

authors： Harris KR,Dighe AS

更新日期：2002-12-01 00:00:00

abstract：:Proteomic studies of cells via surface-enhanced laser desorption/ionization spectrometry (SELDI) analysis have enabled rapid, reproducible protein profiling directly from crude samples. We applied this technique to archival cytology material to determine whether distinct, reproducible protein fingerprints could be ide...

journal_title：American journal of clinical pathology

authors： Fetsch PA,Simone NL,Bryant-Greenwood PK,Marincola FM,Filie AC,Petricoin EF,Liotta LA,Abati A

更新日期：2002-12-01 00:00:00

abstract：:To improve the positive predictive value (PPV) for high-risk human papillomavirus (HR-HPV) in primary screening, DNA ploidy was measured on the same liquid-based sample by image cytometry in 984 cases showing discrepancies between cytology and HR-HPV testing. Of the conflicting results, 14.5% corresponded to a cytolog...

journal_title：American journal of clinical pathology

authors： Lorenzato M,Bory JP,Cucherousset J,Nou JM,Bouttens D,Thil C,Dez F,Evrard G,Quereux C,Birembaut P,Clavel C

更新日期：2002-11-01 00:00:00

abstract：:Five patterns of bone marrow infiltration by non-Hodgkin lymphoma or Hodgkin lymphoma are currently recognized, but a true follicular pattern of bone marrow involvement by follicular lymphoma has not been described. In 260 bone marrow trephine biopsy specimens involved by follicular lymphoma, we identified 12 cases wi...

journal_title：American journal of clinical pathology

authors： Torlakovic E,Torlakovic G,Brunning RD

更新日期：2002-11-01 00:00:00

abstract：:Blastomycosis generally results from inhalation of Blastomyces dermatitidis conidia following exposure to contaminated soil in an endemic area. Primary infections commonly involve the lungs, although secondary dissemination to other body sites may occur. We describe 2 cases of osseous blastomycosis in people living ou...

journal_title：American journal of clinical pathology

authors： Veligandla SR,Hinrichs SH,Rupp ME,Lien EA,Neff JR,Iwen PC

更新日期：2002-10-01 00:00:00

abstract：:The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polymarker (LDLR, GYPA, ...

journal_title：American journal of clinical pathology

authors： Giroti RI,Biswas R,Mukherjee K

更新日期：2002-09-01 00:00:00

abstract：:We report an unusual case of acinar cell cystadenoma of the pancreas in a 52-year-old man treated for pulmonary adenocarcinoma. The lesion, located in the body of the pancreas, was revealed incidentally by abdominal computed tomography during follow-up for a pulmonary neoplasm. A left pancreatectomy was performed. The...

journal_title：American journal of clinical pathology

authors： Chatelain D,Paye F,Mourra N,Scoazec JY,Baudrimont M,Parc R,Flejou JF

更新日期：2002-08-01 00:00:00

abstract：:A survey to determine the need for training in medical mycology was sent to 605 US laboratories. Training needs were determined by comparing actual laboratory mycology practices with recommended practices, documenting the extent of mycology training reported by employees, and asking respondents to specify the fungi th...

journal_title：American journal of clinical pathology

authors： Rosner ER,Reiss E,Warren NG,Shadomy HJ,Lipman HB

更新日期：2002-08-01 00:00:00

abstract：:Lesions of the breast containing extravasated mucin span a continuum from benign mucoceles to invasive mucinous (colloid) carcinoma. It is well known that distinguishing benign from malignant mucinous lesions is difficult infine-needle aspiration material. Whether these lesions also are difficult to distinguish in cor...

journal_title：American journal of clinical pathology

authors： Renshaw AA

更新日期：2002-07-01 00:00:00

abstract：:Measuring HER-2 is important for selecting optimal therapy and predicting prognosis in breast cancer patients. Current methods for evaluating HER-2 include measuring protein overexpression by immunohistochemistry (IHC), measuring gene copy number by fluorescent in situ hybridization (FISH), and measuring shed antigen ...

journal_title：American journal of clinical pathology

authors： Yeh IT

更新日期：2002-06-01 00:00:00

abstract：:The international sensitivity index (ISI) calibration of point-of-care-test (POCT) prothrombin time (PT) whole blood monitors is complex, requiring manual PT testing of 60 patients' and 20 healthy subjects' plasma samples. The possibility of reducing these numbers was studied by a Monte Carlo Bootstrap study for 2 POC...

journal_title：American journal of clinical pathology

authors： Poller L,Keown M,Chauhan N,van den Besselaar AM,Tripodi A,Jespersen J,Shiach C

更新日期：2002-06-01 00:00:00

abstract：:The determination of tumor cell estrogen receptor (ER) expression status by immunohistochemical analysis has become standard practice, yet assay reproducibility has not been assessed adequately. By using a breast cancer tissue microarray, we examined interlaboratory variability in ER reporting. A 2-fold redundant tiss...

journal_title：American journal of clinical pathology

authors： Parker RL,Huntsman DG,Lesack DW,Cupples JB,Grant DR,Akbari M,Gilks CB

更新日期：2002-05-01 00:00:00

abstract：:Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin ...

journal_title：American journal of clinical pathology

authors： Swartz MJ,Batra SK,Varshney GC,Hollingsworth MA,Yeo CJ,Cameron JL,Wilentz RE,Hruban RH,Argani P

更新日期：2002-05-01 00:00:00

abstract：:In 10 cases of Barrett adenocarcinoma, samples from 8 tumor areas (including superficial and deep from peripheral and central areas) and a regional lymph node metastasis were studied for amplification of c-myc, c-erbB-2, and EGFR. We analyzed loss of heterozygosity (LOH) at 3 loci (APC, MCC, and RB) and 2 anonymous mi...

journal_title：American journal of clinical pathology

authors： Owonikoko T,Rees M,Gabbert HE,Sarbia M

更新日期：2002-04-01 00:00:00

abstract：:We used a panel of paraffin antibodies to determine whether neoplastic and nonneoplastic lymphoid aggregates in the bone marrow can be distinguished reliably. Formalin-fixed, paraffin-embedded bone marrow core biopsy specimens with lymphoid aggregates were stained using primary antibodies directed against bcl-2, bcl-6...

journal_title：American journal of clinical pathology

authors： West RB,Warnke RA,Natkunam Y

更新日期：2002-04-01 00:00:00

abstract：:We determined whether there were additional diagnostic findings in additional level sections performed on polyps with no pathologic diagnosis (NPD) or those in which only lymphoid aggregates (LAs) were seen initially and determined the level at which findings were identified. All colorectal biopsy specimens submitted ...

journal_title：American journal of clinical pathology

authors： Nash JW,Niemann T,Marsh WL,Frankel WL

更新日期：2002-03-01 00:00:00